Gene mapping, discovery, and expression : methods and protocols /

Bibliographic Details
Other Authors: Bina, Minou
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2006]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 338.
Subjects:
Table of Contents:
  • Use of genome browsers to locate your favorite genes
  • Methods for identifying and mapping recent segmental and gene duplications in eukaryotic genomes
  • Identification and mapping of paralogous genes on a known genomic DNA sequence
  • Quantitative DNA fiber mapping in genome research and construction of physical maps
  • PRINS for mapping single copy genes
  • Vista family of computational tools for comparative analysis of DNA sequences and whole genomes
  • Computational prediction of cis-regulatory modules from multispecies alignments using Galaxy, Table Browser, and GALA
  • Comparative promoter analysis in vertebrate genomes with the CORG workbench
  • cis-regulatory region analysis using BEARR
  • A database of 9-mers from promoter region of human protein-coding genes
  • A program toolkit for the analysis of regulatory regions of genes
  • Analysis of allele-specific gene expression
  • Construction of micro-RNA containing vectors for expression in mammalian cells
  • Mining microarray data at NCBI's gene expression omnibus (GEO)
  • The Stanford microarray database : a user's guide
  • Detecting nucleosome ladders on unique DNA sequences in mouse liver nuclei
  • DNA methyltransferase probing of DNA-protein interactions
  • Protein binding microarrays (PBMS) for the rapid, high-throughput characterization of the sequence specificities of DNA binding proteins
  • Quantitative profiling of protein-DNA binding on microarrays
  • Analysis of protein-DNA binding by streptavidin-agarose pulldown
  • Isolation and mass spectrometry of specific DNA-binding proteins
  • Isolation of transcription factor complexes by in vivo biotinylation tagging and direct binding to streptavidin beads