Handbook of assay development in drug discovery /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Boca Raton :
CRC/ Taylor & Francis,
2006.
|
| Series: | Drug discovery series ;
5. |
| Subjects: |
Table of Contents:
- Protein kinases in drug discovery : rationale, success, and challenge
- An introduction to the protein tyrosine phosphatase gene family and screening assay development
- Time-resolved fluorescence based assays for kinases
- Development of high-throughput screening assays in scintillating microplates (flashplates) to identify inhibitors of kinase activity
- Development of high-throughput screening assays for kinase drug targets using AlphaScreen technology
- Homogeneous high-throughput screening (HTS) assays for serine/threonine kinases using beta-galactosidase enzyme fagment complementation
- IMAP assays for protein kinases
- A homogeneous, luminescent, high-throughput, versatile assay for a wide range of kinases
- Proteases as drug targets
- A comparison of homogeneous bioluminescent and fluorescent methods for protease assays
- Scintillation proximity assay (SPA) receptor binding assays
- Radioligand binding filtration assay : full automation
- Nuclear receptors as drug targets
- Nuclear receptor scintillation proximity assays
- Homogeneous assay development for nuclear receptor using AlphaScreen technology
- Development of nuclear receptor homogeneous assay using the Lance technology
- The emerging role of cell-based assays in drug discovery
- The preparation of cells for high-content screening
- The evolution of cAMP assays
- A homogeneous, fluorescent polarization assay for inositol 1,4,5-trisphosphate (Ins P₃)
- Scintillation proximity assay of inositol phosphates
- Measuring calcium mobilization with Gq-coupled GPCRs using the fluorometric imaging plate reader (FLIPR)
- Development of FLIPR-based HTS assay for Gi-coupled GPCRs
- Aurora assays
- Membrane potential based assays for ion channels and electrogenic transporters
- Reporter gene assays for drug discovery
- mRNA detection from cells using Quantigene® branched DNA technology
- Homogeneous multiwell assays for measuring cell viability, cytotoxicity, and apoptosis
- Development of image-based assays for drug discovery
- GPCR internalization measured by image analysis
- TRANSFLUOR provides a universal cell-based assay for screening G protein-coupled receptors