Myeloid leukemia : methods and protocols /

Bibliographic Details
Other Authors: Iland, Harry, Hertzberg, Mark, Marlton, Paula
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2006]
Series:Methods in molecular medicine ; 125.
Subjects:
Table of Contents:
  • Isolation of RNA and DNA from leukocytes and cDNA synthesis
  • Cytogenetic and FISH techniques in myeloid malignancies
  • Overview of real-time RT-PCR strategies for quantification of gene rearrangements in the myeloid malignancies
  • Diagnosis and monitoring of chronic myeloid leukemia by qualitative and quantitative RT-PCR
  • Detection of BCR-ABL mutations and resistance to imatinib mesylate
  • Deletion of the derivative chromosome 9 in chronic myeloid leukemia
  • Diagnosis and monitoring of PML-RARA-positive acute promyelocytic leukemia by qualitative RT-PCR
  • Diagnosis and monitoring of PML-RARA-positive acute promyelocytic leukemia by quantitative RT-PCR
  • Diagnosis and monitoring of AML1MTG8 (ETO)-positive acute myeloid leukemia by qualitative and quantitative RT-PCR
  • Diagnosis and monitoring of CBFB-MYH11-positive acute myeloid leukemia by qualitative and quantitative RT-PCR
  • Detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia
  • FLT3 mutations in acute myeloid leukemia
  • WT-1 overexpression in acute myeloid leukemia and myelodysplastic syndromes
  • Classification of AML by DNA-oligonucleotide microarrays
  • Classification of AML using a monoclonal antibody microarray
  • Methods for the detection of the JAK2 V617F mutation in human myeloproliferative disorders
  • Overexpression of PRV-1 gene in polycythemia rubra vera and essential thrombocythemia
  • Chimerism analysis following nonmyeloablative stem cell transplantation