G protein-coupled receptor-protein interactions /

Bibliographic Details
Other Authors: George, Susan R., O'Dowd, Brian Francis, 1950-
Format: Book
Language:English
Published: Hoboken, N.J. : Wiley-Liss, [2005]
Series:Receptor biochemistry and methodology (Unnumbered)
Subjects:
Table of Contents:
  • Purification of the G protein-coupled receptor rhodopsin for structural studies
  • Cooperativity in ligand binding to deduce receptor-receptor interaction
  • Analysis of receptor-receptor interactions using time-resolved fluorescence resonance energy transfer
  • Real-time imaging of GPCR-mediated arrestin translocation as a strategy to evaluate receptor-protein interactions
  • Localization of G protein-coupled receptors and interacting proteins in membrane microdomains
  • Identification of peptide ligand-binding domains with G protein-coupled receptors using photoaffinity labeling
  • Analysis in real-time of the assembly and disassembly of soluble G protein-coupled-receptor-G protein complexes
  • Approaches to studying membrane protein oligomerization : use of cysteine crosslinking strategies to analyze receptor-receptor interactions
  • Co-immunoprecipitation as a strategy to evaluate receptor-receptor or receptor-protein interactions
  • Use of MALDI-TOF and mass spectrometry in the evaluation of receptor-protein interactions
  • Use of the yeast two-hybrid system to detect receptor-protein interactions
  • GST fusion protein and pull-down strategies to evaluate receptor-receptor or receptor-protein interactions
  • G protein fusion to monitor integral membrane protein-protein interactions
  • Enzyme-linked immunosorbent assays as tools in studying interactions of G protein-coupled receptors
  • Electrophysiological characterization of GPCR and ligand-gated channel cross-talk
  • BRET as a strategy to evaluate receptor-receptor or receptor-protein interactions