Cell migration : developmental methods and protocols /

Bibliographic Details
Other Authors: Guan, Jun-Lin
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2005]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 294.
Subjects:
Table of Contents:
  • Cell migration : an overview
  • Boyden chamber assay
  • Wound-healing assay
  • Analysis of cell migration using the Dunn chemotaxis chamber and time-lapse microscopy
  • Cell-adhesion assays
  • Cell-spreading assays
  • Cell-scatter assay
  • Cell migration analyses within fibroblast-derived 3-D matrices
  • Tumor cell invasion assays
  • Analysis of endothelial cell migration under flow
  • Angiogenesis assays in the chick CAM
  • Investigations of neuronal migration in the central nervous sytem
  • Analysis of cell migration in Caenorhabditis elegans
  • Analysis of cell migration using Drosophila as a model system
  • Imaging cell movement during dorsal closure in Drosophila embryos
  • Analysis of cell movements in zebrafish embryos : morphometrics and measuring movement of labeled cell populations in vivo
  • The Xenopus embryo as a model system for studies of cell migration
  • Neural crest migration methods in the chicken embryo
  • In vivo and in vitro models of mammalian angiogenesis
  • Measurement of protein tyrosine phosphorylation in cell adhesion
  • Cell-adhesion assays : fabrication of an E-cadherin substratum and isolation of lateral and basal membrane patches
  • Application of microscope-based FRET to study molecular interactions in focal adhesions of live cells
  • Use of multiphoton imaging for studying cell migration in the mouse
  • Using microfluidic channel networks to generate gradients for studying cell migration