Amyloid proteins : methods and protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2005]
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 299. |
| Subjects: |
Table of Contents:
- Preparation of aggregate-free, low molecular weight amyloid-beta for assembly and toxicity assays
- Determination of peptide oligomerization state using rapid photochemical crosslinking
- In vitro preparation of prefibrillar intermediates of amyloid-beta and α-synuclein
- Purification of recombinant tau protein and preparation of Alzheimer-paired helical filaments in vitro
- Cyclic amplification of protein misfolding and aggregation
- X-ray diffraction studies of amyloid structure
- Molecular electron microscopy approaches to elucidating the mechanisms of protein fibrillogenesis
- Time-lapse atomic force microscopy in the characterization of amyloid-like fibril assembly and oligomeric intermediates
- Fourier transform infrared and circular dichroism spectroscopies for amyloid studies
- Quasielastic light scattering for protein assembly studies
- Intrinsic fluorescent detection of tau conformation and aggregation
- Quantitative measurement of fibrillogenesis by mass spectrometry
- Isolation and culturing of human vascular smooth muscle cells
- Murine cerbrovascular cells as a cell culture model for cerebral amyloid angiopathy : isolation of smooth muscle and endothelial cells from mouse brain
- Purification of human wild-type or variant cystatin C from conditioned media of transfected cells
- Prion propagation in cell culture
- Preparation and propagation of amyloid-enhancing factor
- Purification of amyloid protein AA subspecies from amyloid-rich human tissues
- Purification of transthyretin and transthyretin fragments from amyloid-rich human tissues
- Extraction and chemical characterization of tissue-deposited proteins from minute diagnostic biopsy specimens
- Tissue processing prior to protein analysis and amyloid-beta Quantitation
- ELISA method for measurement of amyloid-beta levels
- Histological staining of amyloid-beta in mouse brains
- The mouse model for scrapie : inoculation, clinical scoring, and histopathological techniques
- Radiolabeling of amyloid-beta peptides
- In vivo imaging amyloid-beta deposits in mouse brain with multiphoton microscopy
- Magnetic resonance imaging of amyloid plaques in transgenic mice