Molecular cardiology : methods and protocols /

Bibliographic Details
Other Authors: Sun, Zhongjie
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2005]
Series:Methods in molecular medicine ; 112.
Subjects:
Table of Contents:
  • Introduction to methods in molecular cardiology
  • Gene therapy approaches to cardiovascular disease
  • Gene therapy for myocardial ischemia using hypoxia inducible double plasmid
  • Cardiac gene delivery using DNA-adenoviral constructs
  • Manipulation of neuronal nitric oxide synthase within the paraventricular nucleus using adenovirus and antisense technology
  • TAT-mediated protein transduction : delivering biologically active proteins into the heart
  • Expression of human eNOS in cardiac and endothelial cells
  • Strategies of conditional gene expression in myocardium : an overview
  • Gene silencing method using adenoviral RNAi vector
  • In cardiomyocytes and vascular smooth muscule cells
  • Cardiac commitment of embryonic cells for myocardial repair
  • Autologous mesenchymal stem cells for post ischemic myocardial repair
  • Cellular therapy with autologous skeletal myoblasts for ischemic heart disease and heart failure
  • Protocols for myocardial infarction repair using fetal cardiac myocytes
  • Methods for examining stem cells in post ischemic and transplanted hearts
  • Endothelial progenitor cell/ex vivo expansion and gene transfer
  • Delineation of sequences essential for specific promoter activation during pressure overloaded hypertrophy or factor-induced hypertrophy
  • Evaluation of cardiac isoform of 2 macroglobulin as a factor inducing cardiac hypertrophy
  • Detection of apoptosis in cardiovascular diseases
  • Detection of cardiac signaling in the injured and hypertrophied heart
  • Affymetrix oligonucleotide analysis of gene expression in the injured heart
  • Identification and validation of loci and candidate genes
  • Linked to cardiac hypertrophy
  • Induction and analysis of cardiac hypertrophy in transgenic animal model
  • Methods for the detection of altered -adrenergic signaling pathways in hypertrophied hearts
  • Transgenic mouse models for cardiac dysfunction by a specific genetic manipulation
  • Cardiomyocyte defects in diabetic models and protection with cardiac-targeted transgenes
  • Protocols for cardiac-specific overexpression of angiotensin II type 1 receptor in transgenic rats