Ubiquitin-proteasome protocols /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2005]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 301. |
| Subjects: |
Table of Contents:
- Small-molecule inhibitors of proteasome activity
- Purification of E1 and E1-like enzymes
- Assays for RING family ubiquitin ligases
- Ubiquitin chain synthesis
- Purification of proteasomes, proteasome subcomplexes, and proteasome-associated proteins from budding yeast
- Recognition and processing of misfolded proteins by PA700, the 19S regulatory complex of the 26S proteasome
- Cell-free assay for ubiquitin-independent proteasomal protein degradation
- Assays of proteasome-dependent cleavage products
- Identification of components of protein complexes
- Mass spectrometric determination of protein ubiquitination
- Reconstitution of endoplasmic reticulum-associated degradation using yeast membranes and cytosol
- Reticulocyte lysate as a model system to study endoplasmic reticulum membrane protein degradation
- Deubiquitinating enzyme purifucation, assay inhibitors, and characterization
- Measuring ubiquitin conjugation in cells
- Assays for proteasome assembly and maturation
- N-terminal ubiquitination
- Quantitating defective ribosome products
- Endoplasmic reticulum-associated protein quality control and degradation : screen for ERAD mutants after ethylmethane sulfonate mutagenesis-- Endoplasmic reticulum-associated protein quality control and degradation : genome wide screen for ERAD components
- Cystic fibrosis transmembrane conductance regulator as a model substrate to study endoplasmic reticulum protein quality control in mammalian cells
- Aggresome formation
- Detection of sumoylated proteins
- Proteasome inhibitors in cancer therapy-- Parkinson's disease : assays for the ubiquitin ligase activity of neural Parkin