Molecular toxicology protocols /

Bibliographic Details
Other Authors:	Keohavong, Phouthone, Grant, Stephen G.
Format:	Book
Language:	English
Published:	Totowa, N.J. : Humana Press, [2005]
Series:	Methods in molecular biology (Clifton, N.J.) ; v. 291.
Subjects:	DNA Adducts > analysis. DNA Mutational Analysis. Apoptosis. DNA Repair.

Table of Contents:

³²P-postlabeling analysis of DNA adducts
Modification of the ³²P-postlabeling method to detect a single adduct species as a single spot
DNA isolation and sample preparation for quantification of adduct levels by accelerator mass spectrometry
Fluoroimaging-based immunoassay of DNA photoproducts in ultraviolet-B-irradiated tadpoles
Analysis of DNA strand cleavage at abasic sites
Premature chromosome condensation in human resting peripheral blood lymphocytes for chromosome aberration analysis using specific whole-chromosome DNA hybridization probes
Mutagen-induced chromatid breakage as a marker of cancer risk
Flow cytometric analysis of micronuclei in erythrocytes
The comet assay : a sensitive genotoxicity test for the detection of DNA damage
Computerized image analysis software for the comet assay
The comet-FISH technique : a tool for detection of specific DNA damage and repair
DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis
Analysis of in vivo mutation in the Hprt and Tk genes of mouse lymphocytes
Quantifying in vivo somatic mutations using transgenic mouse model systems
Methods for detecting somatic mutations in vitro : the human T-cell cloning assay selecting for HPRT mutants
Molecular analysis of mutations in the human HPRT gene
Simultaneous quantification of t (14;18) and HPRT Exon 2/3 deletions in human lymphocytes
The GPA in vivo somatic mutation assay
Flow cytometric measurement of mutant T cells with altered expression of TCR : detecting somatic mutations in humans and mice
Mutation screening of the TP53 gene by temporal temperature gradient gel electrophoresis
Analysis of K-RAS and P53 mutations in sputum samples
Allele-specific competitive blocker-PCR detection of rare base substitution
Gel-based nonradioactive single-strand conformational polymorphism and mutation detection : limitations and solutions
Detection and characterization of oncogene mutations in preneoplastic and early neoplastic lesions
Detection of DNA double-strand breaks and chromosome translocations using ligation-mediated PCR and inverse PCR
Microsatellite instability : an indirect assay to detect defects in the cellular mismatch repair machinery
Unscheduled DNA synthesis : a functional assay for global genomic nucleotide excision repair
Analysis of DNA repair using transfection-based host cell reactivation
An immunoassay for measuring repair of ultraviolet photoproducts
Analysis of DNA double-strand break repair by nonhomologous end joining in cell-free extracts from mammalian cells
Measuring recombination proficiency in mouse embryonic stem cells
Strategies for measurement of biotransformation enzyme gene expression
Genotyping technologies : application to biotransformation enzyme genetic polymorphism screening
TaqMan flurogenic detection system to analyze gene transcription in autopsy material
Development of quantitative reverse transcriptase PCR assays for measuring gene expression
Quantification of selective phosphatidylserine oxidation during apoptosis
Quantitative method of measuring phophatidylserine externalization during apoptosis using electron paramagnetic resonance spectroscopy and annexin-conjugated iron
Detection of programmed cell death in cells exposed to genotoxic agents using a caspase activation assay