Fly pushing : the theory and practice of Drosophila genetics /
| Main Author: | |
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| Format: | Book |
| Language: | English |
| Published: |
Cold Spring Harbor, N.Y. :
Cold Spring Harbor Laboratory Press,
[2004]
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| Edition: | Second edition. |
| Subjects: |
Table of Contents:
- The basics of doing a cross
- Fly chromosomes
- Recognizing markers
- Nomenclature
- Evolution of gene names
- What makes flies so great? Balancer chromosomes
- Deciphering mating schemes
- Basic fly husbandry
- Coddling difficult strains
- Collecting flies for crosses
- Isolating new variants
- Principles
- Mutagens
- Ethylmethanesulfonate
- Ethylnitrosourea
- Radiation
- Insertional mutagenesis
- Genetic schemes for detecting mutants
- New alleles at an autosomal locus
- New alleles on the X
- Identifying new genes by mutagenesis
- Isogenizing the starter stock
- The mutagenesis screen
- Screening the X for new genes
- Insertional mutagenesis and enhancer trapping
- Enhancer-trap screens
- New insertional alleles of known loci
- Mutating genes for which there are no allelic differences
- Screening over a deletion of the locus
- Targeted insertional mutagenesis
- Homologous recombination
- RNA interference
- Searching for gain of function
- Generating mutants : one size does not fit all
- Mapping
- Segregation analysis
- Problems with balancers
- Meiotic mapping
- Deletion mapping
- Duplication mapping
- Synthesizing deletions and duplications
- Identifying new embryonic genes by deletion mapping
- Mapping P-element insertion sites
- Fine-grain traditional meiotic mapping with P-element markers
- Mapping by P-element-induced male recombination
- Fine-grain mapping by transposon induced deletions
- Mapping by DNA polymorphisms
- Synthesizing specific genotypes
- Principles
- Simple manipulations of a single chromosome
- Linking mutations
- Manipulating two chromosomes
- Pitfalls
- How many flies?
- Which sex?
- Manipulating three chromosomes
- Making rearrangements
- Inducing deletions
- Synthesizing stable deletions from T(Y;A)s
- Synthesizing deletions from P-element insertions
- Inducing duplications
- Synthesizing stable duplications from T(Y;A)s
- Synthesizing attached-Xs
- Autosynaptic chromosomes and the joys of gibberish
- Analysis of mutations : I. characteristics of the allele
- Formal classification of mutations
- Principles
- Null alleles (amorphs)
- Hypomorphs
- Hypermorphs
- Neomorphs
- Antimorphs
- Gain versus loss of function
- Intragenic interactions
- Interacting loci
- Ordering genes in a pathway
- Dosage-dependent interactions
- Conditional alleles
- Inducible promoters
- Maintenance of mutant phenotypes
- Genetic background
- Analysis of mutations : II. mosaics
- Principles
- Chromosome loss
- Uses of chromosome loss mosaics
- Fate mapping
- Focus of gene action
- Methods for inducing chromosome loss
- Ring loss
- Chromosome loss mosaics for autosomal genes
- paternal-loss (pal)
- claret-non-disjunctional (cand)
- Care of mosaic-producing stocks
- Mitotic recombination
- Synchronizing age of cultures
- Uses of mitotic recombination mosaics
- Lineage analysis
- Focus of gene action
- Time of gene action
- Methods for producing mitotic recombinant clones
- Markers for internal tissues
- GAL4 and expression mosaicism
- Cell ablations
- GAL4 targeted FLP/FRT recombination
- Isolating tissue-targeted mutations using mitotic recombination
- P-element replacement for exploitation of existing enhancer traps
- Commencement
- Solutions to problems
- Glossary
- Appendix : Fly resources