http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
the theory and practice of Drosophila genetics
The basics of doing a cross -- Fly chromosomes -- Recognizing markers -- Nomenclature -- Evolution of gene names -- What makes flies so great? Balancer chromosomes -- Deciphering mating schemes -- Basic fly husbandry -- Coddling difficult strains -- Collecting flies for crosses -- Isolating new variants -- Principles -- Mutagens -- Ethylmethanesulfonate -- Ethylnitrosourea -- Radiation -- Insertional mutagenesis -- Genetic schemes for detecting mutants -- New alleles at an autosomal locus -- New alleles on the X -- Identifying new genes by mutagenesis -- Isogenizing the starter stock -- The mutagenesis screen -- Screening the X for new genes -- Insertional mutagenesis and enhancer trapping -- Enhancer-trap screens -- New insertional alleles of known loci -- Mutating genes for which there are no allelic differences -- Screening over a deletion of the locus -- Targeted insertional mutagenesis -- Homologous recombination -- RNA interference -- Searching for gain of function -- Generating mutants : one size does not fit all -- Mapping -- Segregation analysis -- Problems with balancers -- Meiotic mapping -- Deletion mapping -- Duplication mapping -- Synthesizing deletions and duplications -- Identifying new embryonic genes by deletion mapping -- Mapping P-element insertion sites -- Fine-grain traditional meiotic mapping with P-element markers -- Mapping by P-element-induced male recombination -- Fine-grain mapping by transposon induced deletions -- Mapping by DNA polymorphisms -- Synthesizing specific genotypes -- Principles -- Simple manipulations of a single chromosome -- Linking mutations -- Manipulating two chromosomes -- Pitfalls -- How many flies? -- Which sex?
Manipulating three chromosomes -- Making rearrangements -- Inducing deletions -- Synthesizing stable deletions from T(Y;A)s -- Synthesizing deletions from P-element insertions -- Inducing duplications -- Synthesizing stable duplications from T(Y;A)s -- Synthesizing attached-Xs -- Autosynaptic chromosomes and the joys of gibberish -- Analysis of mutations : I. characteristics of the allele -- Formal classification of mutations -- Principles -- Null alleles (amorphs) -- Hypomorphs -- Hypermorphs -- Neomorphs -- Antimorphs -- Gain versus loss of function -- Intragenic interactions -- Interacting loci -- Ordering genes in a pathway -- Dosage-dependent interactions -- Conditional alleles -- Inducible promoters -- Maintenance of mutant phenotypes -- Genetic background -- Analysis of mutations : II. mosaics -- Principles -- Chromosome loss -- Uses of chromosome loss mosaics -- Fate mapping -- Focus of gene action -- Methods for inducing chromosome loss -- Ring loss -- Chromosome loss mosaics for autosomal genes -- paternal-loss (pal) -- claret-non-disjunctional (cand) -- Care of mosaic-producing stocks -- Mitotic recombination -- Synchronizing age of cultures -- Uses of mitotic recombination mosaics -- Lineage analysis -- Focus of gene action -- Time of gene action -- Methods for producing mitotic recombinant clones -- Markers for internal tissues -- GAL4 and expression mosaicism -- Cell ablations -- GAL4 targeted FLP/FRT recombination -- Isolating tissue-targeted mutations using mitotic recombination -- P-element replacement for exploitation of existing enhancer traps -- Commencement -- Solutions to problems -- Glossary -- Appendix : Fly resources
Includes bibliographical references (pages 171-182) and index.
0879697113 (concealed wire-o : alk. paper)