Bioconjugation protocols : strategies and methods /

Bibliographic Details
Other Authors: Niemeyer, Christof M.
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2004]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 283.
Subjects:
Table of Contents:
  • Antibody and enzyme conjugates
  • Streptavidin-biotin crosslinking of therapeutic enzymes with carrier antibodies : nanoconjugates for protection against endothelial oxidative stress
  • Characterization of endothelial internalization and targeting of antibody-enzyme conjugates in cell cultures and in laboratory animals
  • Smart polymer-streptavidin conjugates
  • Conjugates of peptides and proteins to polyethylene glycols
  • Chemical productin of bispecific antibodies
  • Preparation in immunoconjugates using antibody oligosaccharide moieties
  • Synthesis of hapten-protein conjugates using microbial transglutaminase
  • Nucleic acid conjugates
  • Flourescent sample labeling for DNA microarray analyses
  • High-density labeling of DNA for single molecule sequencing
  • Sequence-specific DNA labeling using methyltransferases
  • Hapten labeling of nucleic acids for immuno-polymerase chain reaction applications
  • Covalent coupling of DNA oligonucleotides and streptavidin
  • Synthesis of oligonucleotide-peptide and oligonucleotide-protein conjugates
  • Synthesis of peptide nucleic acid-peptide conjugates
  • Glycosyl and lipid conjugates
  • Protein lipidation
  • Synthesis of lipidated peptides
  • In vitro semisynthesis and application of C-terminally modified rab proteins
  • Generation and characterization of ras lipoproteins based on chemical coupling
  • Conjugation of glycopeptide thioesters to expressed protein fragments : semisynthesis of Glycosylated Interleukin-2
  • Subtilisin-catalyzed glycopeptide condensation
  • Biofunctionalization of surfaces
  • Peptide nucleic acid microarrays
  • Synthesis and characterization of deoxyribonucleic acid-conjugated gold nanoparticles
  • Biofunctionalization of carbon nanotubes for atomic force microscopy imaging