Epigenetics protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2004]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 287. |
| Subjects: |
Table of Contents:
- Methods of epigenetic analysis
- Chromatin immunoprecipitation assays
- Native chromatin immunoprecipitation
- Q-PCR in combination with ChIP assays to detect changes in chromatin acetylation
- Restriction endonuclease accessibility as a determinant of altered chromatin structure
- Measuring changes in chromatin using micrococcal nuclease
- DNasel hypersensitivity analysis of chromatin structure
- Inhibition of histone deacetylases
- Site-specific analysis of histone methylation and acetylation
- Analysis of mammalian telomere position effect
- Activity assays for poly-ADP ribose polymerase 1
- Multigenerational selection and detection of altered histone acetylation and methylation patterns : toward a quantitative epigenetics in Drosophila
- Profiling DNA methylation by bisulfite genomic sequencing : problems and solutions
- Methylation-sensitive single-strand conformation analysis : a rapid method to screen for and analyze DNA methylation
- SIRPH analysis : SNuPE with IP-RP-HPLC for quantitative measurements of DNA methylation at specific CpG sites
- Real-time PCR-based assay for quantitative determination of methylation status
- Denaturing gradient gel electrophoresis to detect methylation changes in DNA
- Photocrosslinking oligonucleotide hybridization assay for concurrent gene dosage and CpG methylation analysis
- Methylation-specific oligonucleotide microarray
- Methylation-specific PCR in situ hybridization
- Realtive quantitation of DNA methyltrasnferase mRNA by real-time RT-PCR assay
- DMB (DNMT-Magnetic Beads) assay : measuring DNA methyltransferase activity in vitro