Protein NMR techniques /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2004]
|
| Edition: | Second edition. |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 278. |
| Subjects: |
Table of Contents:
- Screening and optimizing protein production in E. coli
- Isotopic labelling of recombinant proteins from the methylotrophic yeast Pichia pastoris
- Perdeuteration/site-specific protonation approaches for high moleculae weight proteins
- Segmental isotopic labelling for structural biological applications of NMR
- TROSY-based correlation and NOE spectroscopy for NMR structural studies of large proteins
- Media for studies of partially aligned states
- Residual dipolar couplings in protein structure determination
- Projection angle restraints for studying structure and dynamics of biomolecules
- Characterizing domain interfaces by NMR
- Characterization of the overall rotational diffusion of a protein from 15N relaxation measurements and hydrodynamic calculations
- TROSY-based NMR experiments for the study of macromolecular dynamics and hydrogen bonding
- Measurement of intermediate exchange phenomena
- NMR studies of partially folded molten globule states
- Structure determination of protein complexes by NMR
- NMR studies of protein/nucleic acid interactions
- Using NMRView to visualize and analyze the NMR spectra of macromolecules
- Automated NMR structure calculation with CYANA
- NOE assignment with ARIA 2.0: the nuts and bolts
- Membrane protein structure determinations using solid state NMR