Germ cell protocols /
Veterinary embryology techniques.
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2004-]
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 253. Methods in molecular biology (Clifton, N.J.) ; v. 254. |
| Subjects: |
Table of Contents:
- v. 1. Sperm and oocyte analysis
- Cryopreservation of semen of the salmonidae with special reference to large-scale fertilization
- Fertilization and sperm chemotaxis in ascidians
- Assays for vertebrate sperm chemotaxis
- Stallion spermatozoa viability : comparison of flow cytometry with other methods
- Visualization of sperm accessory structures in the mammalian spermatids, spermatozoa, and zygotes by immunofluorescence, confocal, and immunoelectron microscopy
- Localization of zona pellucida receptors on live sperm by fluorophore-conjugated solubilized zone pellucida proteins
- Detection of human papillomavirus DNA in sperm using polymerase chain reaction
- Spermatogonial stem cells
- Molecular and cellular approaches for establishment of an in vitro spermatogenesis system
- Acrosin activity assay for the evaluation of mammalian sperm acrosome reaction
- Isolation of sea urchin sperm plasma membranes
- Detection of centrosome structure in fertilized and artificially activated sea urchin eggs using immunofluorescence microscopy and isolation of centrosomes followed by structural characterization with field emission scanning electron microscopy
- Microscopic techniques for studying sperm-oocyte interaction during fertilization and early embryo development
- Immunocytochemical staining of the metaphase II spindle in mammalian oocytes
- Spindle imaging in living mammalian oocytes using the liquid-crystal polarized light microscope and its practical use
- In vitro maturation and fertilization of pig oocytes
- In vitro maturation and fertilization of human oocytes
- In vitro maturation and fertilization of canine oocytes
- Detection and measurement of membrane-bound protein tyrosine kinases in the zebrafish egg
- A single-cell method for estimation of protein tyrosine kinases in the zebrafish egg
- Activity of MAPK/p90rsk during fertilization in mice, rats, and pigs
- v. 2. Molecular embryo analysis, live imaging, transgenesis, and cloning
- Activity of the germline-specific Oct4-GFP transgene in normal and clone mouse embryos
- Analysis of germline chromatin silencing by double-stranded RNA-mediated interference (RNAi) in Caenorhabditis elegans
- cDNA subtraction and cloning in the field of trophoblast/placental development
- PCR-based cloning and differential screening of RNAs from xenopus primordial germ cells : cloning uniquely expressed RNAs from rare cells
- Differentiating embryonic stem cells into embryoid bodies
- The derivation of murine embryonic stem and embryonic germ cells by selective ablation
- Using multiphoton excitation to explore the murky depths of developing embryos
- Dual-channel confocal rationing of calcium dynamics in living eggs and oocytes
- A method for producing cloned pigs by using somatic cells as donors
- Pronuclear microinjection of mouse zygotes
- Parthenogenesis and nuclear transfer in rabbit oocytes
- Nuclear transfer in cattle
- In vitro fertilization and embryo transfer in felids
- Production of chimeric chickens
- Production of haploid and diploid androgenetic zebrafish
- Construction and detection of flourescent, germline transgenic zebrafish
- Production of zebrafish germline chimeras from cultured cells
- Studying nuclear reprogramming with cell hybrids
- Cytoplastic transfer in mammalian eggs and embryos
- Conventional technologies for cryopreservation of human oocytes and embryos
- Vitrifying and warming of human oocytes, embryos, and blastocysts : vitrification procedures as an alternative to conventional cryopreservation
- Microfluidic removal of cumulus cells from mammalian zygotes
- Mammalian embryo culture in a microfluidic device
- Application of alginate gels to the study of mammalian development
- Using TUNEL in combination with an active caspase-3 immunoassay to identify cells undergoing apoptosis in preimplantation mammalian embryos