Drosophila cytogenetics protocols /

Bibliographic Details
Other Authors: Henderson, Daryl S.
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2004]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 247.
Subjects:
Table of Contents:
  • The chromosomes of Drosophila melanogaster
  • Spermatogenesis : analysis of neiosis and morphogenesis
  • Time-lapse imaging of male meiosis by phase-contrast and fluorescence microscopy
  • Immunocytological analysis of oogenesis
  • Cytological analysis of oogenesis
  • Polytene chromosomes from ovarian nurse cells of Drosophila melanogaster otu mutants
  • Feulgen-DNA cytophotometry for estimating C values
  • Fluorescent BrdU labeling and nuclear flow sorting of the Drosophila ovary
  • Studying nuclear organization in embryos using antibody tools
  • Whole-mount fluorescence In situ hybridization to chromosomes of embryos
  • Orcein staining and the identification of polytene chromosomes
  • Salivary chromosome analysis of aberrations
  • In situ hybridization to polytene chromosomes
  • Combined immunostaining and FISH analysis of polytene chromosomes
  • Electron microscopy of polytene chromosomes
  • Analysis of mitosis in squash preparations of larval brains : Orcein, Giemsa, Hoechst 33258, DAPI, Quinacrine, and N-banding
  • Analyzing chromosome function by high frequency formation of dicentric chromosomes in vivo
  • Analysis of chromosomes of the larval CNS by FISH and BrdU labeling
  • Immunostaining of squash preparations of chromosomes of larval brains
  • Visualizing mitosis in whole-mount larval brains
  • Immunostaining of whole-mount imaginal discs
  • Wing somatic mutation and recombination test
  • Analysis of histoblasts
  • Visualizing apoptosis
  • RNAi in cultured Drosophila cells