A laboratory guide to the mammalian embryo /
| Other Authors: | , , |
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| Format: | Book |
| Language: | English |
| Published: |
Oxford, New York :
Oxford University Press,
2004.
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| Subjects: |
Table of Contents:
- Follicle development in vitro
- Preparation of gametes, in vitro maturation, in vitro fertilization, and embryo recovery and transfer
- Culture of the mammalian preimplantation embryo
- Assessment of preimplantation embryo development and viability
- Micromanipulation : biopsy
- Analysis of intracellular ions in embryos : pH and calcium
- Assesment of nutrient uptake, metabolite production, and enzyme activity
- Metabolic pathway activity
- Confocal imaging of structural molecules in mammalian gametes
- Flourescent in situ hybridisation for detection of aneuploidy in single human blastomeres for preimplantation genetic diagnosis
- Assesment of ploidy, telomere length, and telomerase activity in oocytes and embryos
- Microscale RNA isolation from mammalian embryos
- Relative mRNA transcript abundance in early embryos by reverse transcription-polymerase chain reaction
- Characterization of novel genes during early development by application of differential display RT-PCR
- Two-dimensional protein gel database analysis of embryos, oocytes, and oocyte-associated granulosa cells
- Antisense disruption of gene expression in the preimplantation embryo
- Gene subtraction and analysis
- Analysis of apoptosis in the preimplantation embryo
- Cytoplasmic signaling and cell control in the mouse egg and embryo
- Human embryonic stem cells
- Spermatogonial transplantation
- Somatic cell nuclear transfer
- Cryobiology : slow freezing vitrification embryos