HPLC of peptides and proteins : methods and protocols /

Bibliographic Details
Other Authors: Aguilar, Marie-Isabel
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2004]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 251.
Subjects:
Table of Contents:
  • pt. I. MODES OF HPLC
  • HPLC of Peptides and Proteins: Basic Theory and Methodology
  • Reversed Phase High Performance Liquid Chromatography
  • Ion-Exchange Chromatography
  • High Performance Hydrophobic Interaction Chromatography
  • Gel Filtration Chromatography
  • Hydrophilic Interaction Chromatography
  • Immobilized Metal Ion Affinity Chromatography of Proteins
  • Immunoaffinity Chromatography of Proteins
  • Liquid Chromatography: Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins
  • pt. II. PREPARATIVE METHODOLOGIES
  • Capillary Separations
  • Micropreparative HPLC of Peptides and Proteins
  • Multidimensional HPLC Purification of Proteins
  • Analytical High Performance Liquid Chromatography
  • Prep/Semi-prep Separations of Peptides
  • Large Scale Protein Chromatography
  • pt. III. APPLICATIONS
  • Proteolytic Peptide Mapping
  • Mass Spectrometric Characterization of Post-Translationally Modified Proteins-Phosphorylation
  • Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography /Mass Spectrometry and Liquid Chromatography/Tandem Mass Spectrometry
  • The Use of HPLC in the Analysis of Peptide Metabolism
  • Isolation and Characterization of Naturally Processed MHC-Bound Peptides from the Surface of Antigen Presenting Cells
  • Australian Funnel-Web Spider Venom Analyzed Wirh On-Line RP-HPLC Techniques
  • HPLC and Mass Spectrometry of Intrinsic Membrane Proteins
  • IgG Purification
  • DNA Binding Proteins: Use of LC-MS to Identify Key Domains in RNA Polymerase-Promoter Interactions
  • Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC
  • Automated Versus Manual Peptide Libraries Profiling by Mass Spectrometry
  • Proteome Analysis