Vaccinia virus and poxvirology : methods and protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2004]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 269. |
| Subjects: |
Table of Contents:
- Working safely with vaccinia virus : laboratory technique and the role of vaccinia vaccination
- Construction and isolation of recombinant vaccinia virus using genetic markers
- Construction of recombinant vaccinia virus : cloning into the thymidine kinase locus
- Transient and inducible expression of vaccinia/T7 recombinant viruses
- Construction of recombinant vaccinia viruses using leporipoxvirus catalyzed recombination and reactivation of orthopoxvirus DNA
- Construction of CDNA libraries in vaccinia virus
- Construction and isolation of recombination MVA
- Growing poxviruses and determining virus titer
- Rapid preparation of vaccinia virus DNA template for analysis and cloning by PCR
- Orthopoxvirus diagnostics
- An in vitro transcription system for studying vaccinia virus early genes
- An in vitro transcription system for studying vaccinia virus late genes
- Studying vaccinia virus RNA processing in vitro
- Methods for analysis of poxvirus DNA replication
- Studying the binding and entry of the intracellular and extracellular enveloped forms of vaccinia virus
- Pox, dyes, and videotape : making movies of GFP labeled vaccinia virus
- Interaction analysis of viral cytokine-binding proteins : using surface plasmon resonance
- Monitoring of human immunological responses to vaccinia virus
- Vaccinia virus as a tool for immunologic studies
- Mouse models for studying orthopoxvirus respiratory infections
- Viral glycoprotein-mediated cell fusion assays using vaccinia virus vectors
- Use of dual recombinant vaccinia virus vectors to assay viral glycoprotein-mediated fusion with transfection-resistant primary cell targets
- Poxvirus bioinformatics
- Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens