G protein signaling : methods and protocols /

Bibliographic Details
Other Authors:	Smrcka, Alan V.
Format:	Book
Language:	English
Published:	Totowa, N.J. : Humana Press, [2004]
Series:	Methods in molecular biology (Clifton, N.J.) ; v. 237.
Subjects:	GTP-Binding Proteins > analysis. GTP-Binding Protein Regulators. Genetic Techniques. Clinical Laboratory Techniques. Laboratory Manual.

Table of Contents:

Purification of G protein subunits and G protein effectors
Assays for G protein-dependent effector activation in vitro
Assays for G protein-coupled receptor-mediated and G protein subunit-mediated effector activation in intact cells
Receptor mechanisms, activity, and localization
Disruption of endogenous G protein-signaling systems to understand the role of G protein subunits in intact systems
Posttranslational modifications of regulators of G protein signaling
Use of GFP-tagged G proteins and effectors in intact cells