Cytokine protocols /

Bibliographic Details
Other Authors:	Ley, Marc de
Format:	Book
Language:	English
Published:	Totowa, N.J. : Humana Press, [2004]
Series:	Methods in molecular biology (Clifton, N.J.) ; v. 249.
Subjects:	Cytokines > Laboratory Manuals. Immunologic Techniques > Laboratory Manuals.

Table of Contents:

Large-scale generation of plasmids that express type I interferon
Indentification of trans-acting factors by electrophoretic mobility shaft assay
Modulation of the interferon-γ signal transfection of cells with an antisense-RNA expressing vector
Competitive RT-PCR to quantify small amounts of mRNA
In situ hybridization for cytokines in human tissue biopsies
Purification, identification, and synthesis of chemokines
Receptor isolation and characterization : from protein to gene
Crystallization of cytokine-receptor complexes
Biosensor analysis of receptor-ligand interactions
Analysis of SH2 ligands and identification of sites of interaction
Assays for antiviral activity
Assays for cytotoxicity