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Proteomics in practice : a laboratory manual of proteome analysis /

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Bibliographic Details
Main Author: Westermeier, Reiner
Other Authors: Naven, Tom
Format: eBook
Language:English
Published: Weinheim : Wiley-VCH, [2002]
Subjects:
Proteome.
Proteins > analysis.
Protein Binding.
Gene Expression.
Amino Acid Sequence.
Laboratory Manuals.
Ebooks.
Electronic books.
Online Access:Connect to the full text of this electronic book.
Table of Contents:
  • Expression proteomics
  • Equipment, consumables, reagents
  • Step1 : Sample preparation
  • Step 2 : Isoelectric focusing
  • Step 3 : SDS polyacrylamide gel electrophoresis
  • Step 4 : Staining of the gels
  • Step 5 : Scanning of gels and image analysis
  • Step 6 : Fluorescence difference gel electrophoresis
  • Step 7 : Spot excicion
  • Step 8 : Sample destaining
  • Step 9 : In-gel digestion
  • Step 10 : Microscale desalting and concentration of sample
  • Step 11 : Chemical derivatisation of the peptide digest
  • Step 12 : MS analysis
  • Step 13 : Calibration of the MALDI-ToF MS
  • Step 14 : Preparing for a database search
  • Step 15 : PMF database search unsuccessful.