Cell migration in inflammation and immunity : methods and protocols /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2004]
|
| Series: | Methods in molecular biology.
v. 239. |
| Subjects: |
Table of Contents:
- Chemotaxis and interaction with vascular or lymphatic endothelium
- Analysis of integrin-dependent rapid adhesion under laminar-flow conditions
- Posttranslational processing of chemokines
- Chemotactic profiling of lymphocyte subpopulations
- Measurements of the levels of polymerized actin (F-Actin) in chemokine-stimulated lymphocytes and GFP-coupled cDNA transfected lymphoid cells by flow cytometry
- Evaluation of Rho family small G-protein activity induced by integrin ligation on human leukocytes
- Reconstructing leukocyte migration in 3D extracellular matrix by time-lapse videomicroscopy and computer-assisted tracking
- Analyzing chemotaxis using Dictyostelium discoideum as a model system
- Conditional transgenic models to study chemokine biology
- Intravital microscopy as a tool for studying recruitment and chemotaxis
- Tracking antigen-specific lymphocytes in vivo
- Analysis of homing-receptor expression on infiltrating leukocytes in disease states
- Interaction of viral chemokine inhibitors with chemokines
- Discovery of small-molecule antagonists of chemokine receptors : screening strategy and assays
- Visualization and analysis of adhesive events in brain microvessels by using intravital microscopy
- Animal models to study chemokine receptor function in vivo : mouse models of allergic airway inflammation
- Assessing the role of multiple phosphoinositide 3-kinases in chemokine signaling : use of dominant negative mutants controlled by a tetracycline-regulated gene expression system
- In vitro and in vivo models to study chemokine regulation of angiogenesis
- Real time in vitro assay for studying chemoattractant-triggered leukocyte transendothelial migration under physiological flow conditions
- Generation of monoclonal antibodies against chemokine receptors
- Detection of high-affinity α4-integrin upon leukocyte stimulation by chemoattractants or chemokines