Directed enzyme evolution : screening and selection methods /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2003]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 230. |
| Subjects: |
Table of Contents:
- Genetic complementation protocols
- Use of pol I-deficient E. coli for functional complementation of DNA polymerase
- Selection of novel eukaryotic DNA polymerases by mutagenesis and genetic complementation of yeast
- Autogene selections
- Selection for soluble proteins via fusion with chloramphenicol acetyltransferase
- Proside : a phage-based method for selecting thermostable proteins
- Minimization of proteins by random fragmentation and selection
- Evaluating a screen and analysis of mutant libraries
- Screening mutant libraries in Saccharomyces cerevisiae
- Solid-phase screening using digital image analysis
- Screening for thermostability
- High-throughput screening of mutant α-amylase libraries for increased activity at 120⁰C
- High-throughput carbon monoxide binding assay for cytochromes P450
- High-throughput screen for aromatic hydroxylation
- Colorimetric screen for aliphatic hydroxylation by cytochrome P450 using p-nitrophenyl-substituted alkanes
- High-throughput screens based on NAD(P)H depletion
- High-throughput tetramethylbenzidine (TMB) screen for peroxidases
- Screen for oxidases by detection of hydrogen peroxide with horseradish peroxidase
- Colorimetric dehydrogenase screen board on NAD(P)H generation
- Colorimetric assays for screening laccases
- pH sensing agar plate assays for esterolytic enzyme activity
- A pH-indicator-based screen for hydrolytic haloalkane dehalogenase
- Detection of aromatic α-hydroxyketones with tetrazolium salts
- Selection of heat-stable Clostridium cellulovorans cellulases
- Screening and selection strategies for disulfide isomerase activity
- An overview of high-throughput screening systems for enantioselective enzymatic transformations
- Select protocols of high-throughput ee-screening systems for enantioselective enzymatic transformations
- Directed evolution of the substrate specificities of a site-specific recombinase and an aminoacyl-tRNA synthetase using fluorescence-activated cell sorting (FACS)
- Calmodulin-tagged phage and two-filter sandwich assays for the identification of enzymatic activities
- High-throughput FACS method for directed evolution of substrate specificity
- Improving protein folding efficiency by directed evolution using the GFP folding reporter