http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
xv, 383 pages : illustrations (some color) ; 24 cm.
Genetic complementation protocols -- Use of pol I-deficient E. coli for functional complementation of DNA polymerase -- Selection of novel eukaryotic DNA polymerases by mutagenesis and genetic complementation of yeast -- Autogene selections -- Selection for soluble proteins via fusion with chloramphenicol acetyltransferase -- Proside : a phage-based method for selecting thermostable proteins -- Minimization of proteins by random fragmentation and selection -- Evaluating a screen and analysis of mutant libraries -- Screening mutant libraries in Saccharomyces cerevisiae -- Solid-phase screening using digital image analysis -- Screening for thermostability -- High-throughput screening of mutant α-amylase libraries for increased activity at 120⁰C -- High-throughput carbon monoxide binding assay for cytochromes P450 -- High-throughput screen for aromatic hydroxylation -- Colorimetric screen for aliphatic hydroxylation by cytochrome P450 using p-nitrophenyl-substituted alkanes -- High-throughput screens based on NAD(P)H depletion -- High-throughput tetramethylbenzidine (TMB) screen for peroxidases --Screen for oxidases by detection of hydrogen peroxide with horseradish peroxidase -- Colorimetric dehydrogenase screen board on NAD(P)H generation -- Colorimetric assays for screening laccases -- pH sensing agar plate assays for esterolytic enzyme activity -- A pH-indicator-based screen for hydrolytic haloalkane dehalogenase -- Detection of aromatic α-hydroxyketones with tetrazolium salts -- Selection of heat-stable Clostridium cellulovorans cellulases -- Screening and selection strategies for disulfide isomerase activity -- An overview of high-throughput screening systems for enantioselective enzymatic transformations -- Select protocols of high-throughput ee-screening systems for enantioselective enzymatic transformations -- Directed evolution of the substrate specificities of a site-specific recombinase and an aminoacyl-tRNA synthetase using fluorescence-activated cell sorting (FACS) -- Calmodulin-tagged phage and two-filter sandwich assays for the identification of enzymatic activities -- High-throughput FACS method for directed evolution of substrate specificity -- Improving protein folding efficiency by directed evolution using the GFP folding reporter
edited by Frances H. Arnold and George Georgiou.
Includes bibliographical references and index.
Methods in molecular biology (Clifton, N.J.) ; v. 230