Diabetes mellitus : methods and protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana,
[2003]
|
| Series: | Methods in molecular medicine ;
83. |
| Subjects: |
Table of Contents:
- Isolation of islets of Langerhans from rodent pancreas
- Purification of rat pancreatic beta-cells by fluorescence-activated cell sorting
- Assessment of insulin secretion in the mouse
- Detection of insulin production by immunohistochemistry
- Quantification of the level of insulin gene expression
- Chromatin immunoprecipitation using isolated islets of Langerhans
- Adenoviral gene transfer into beta-cell lines
- Utilization of NOD mice in the study of type 1 diabetes
- Introduction of DNA into 3T3-L1 adipocytes by electroporation
- Analysis of insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes
- Fractionation analysis of the subcellular distribution of GLUT-4 in 3T3-L1 adipocytes
- Visualization and quantitation of integral membrane sheet assay
- Assaying tyrosine phosphorylation of insulin receptor and insulin receptor substrates
- Measuring insulin-stimulated phosphatidyl-inositol 3-kinase activity
- Assaying AKT/protein kinase B activity
- Measurements of cellular phosphoinositide levels in 3T3-L1 adipocytes
- Measurement of glycogen synthesis and glycogen synthase activity in 3T3-1 adipocytes
- Measurement of contraction-stimulated GLUT-4 translocation in isolated skeletal muscle
- Single embryo measurement of basal- and insulin-stimulated glucose uptake
- Immunohistologic staining of muscle and embryos to detect insulin-stimulated translocation of glucose transporters