http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Isolation of islets of Langerhans from rodent pancreas -- Purification of rat pancreatic beta-cells by fluorescence-activated cell sorting -- Assessment of insulin secretion in the mouse -- Detection of insulin production by immunohistochemistry -- Quantification of the level of insulin gene expression -- Chromatin immunoprecipitation using isolated islets of Langerhans -- Adenoviral gene transfer into beta-cell lines -- Utilization of NOD mice in the study of type 1 diabetes -- Introduction of DNA into 3T3-L1 adipocytes by electroporation -- Analysis of insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes -- Fractionation analysis of the subcellular distribution of GLUT-4 in 3T3-L1 adipocytes -- Visualization and quantitation of integral membrane sheet assay -- Assaying tyrosine phosphorylation of insulin receptor and insulin receptor substrates -- Measuring insulin-stimulated phosphatidyl-inositol 3-kinase activity -- Assaying AKT/protein kinase B activity -- Measurements of cellular phosphoinositide levels in 3T3-L1 adipocytes -- Measurement of glycogen synthesis and glycogen synthase activity in 3T3-1 adipocytes -- Measurement of contraction-stimulated GLUT-4 translocation in isolated skeletal muscle -- Single embryo measurement of basal- and insulin-stimulated glucose uptake -- Immunohistologic staining of muscle and embryos to detect insulin-stimulated translocation of glucose transporters
Includes bibliographical references and index.