E. coli plasmid vectors : methods and applications /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2003]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 235. |
| Subjects: |
Table of Contents:
- Function and organization of plasmids
- Choosing a cloning vector
- Escherichia coli host strains
- Chemical transformation of E. coli
- Electroporation of E. coli
- DNA transfer by bacterial conjugation
- Cosmid packaging and infection of E. coli
- Isolation of plasmids from E. coli by boiling lysis
- High-purity plasmid isolation using silica oxide
- High-throughput plasmid extraction using microtiter plates
- Isolation of cosmid and BAC DNA from E. coli
- Preparation of single-stranded DNA from phagemid vectors
- Using desktop cloning software to plan, track, and evaluate cloning projects
- Cloning in plasmid vectors
- Extraction of DNA from agarose gels
- Cloning PCR products with T-vectors
- Rapid screening of recombinant plasmids
- Restriction analysis of recombinant plasmids
- Screening recombinant DNA libraries
- Sequencing using fluorescent-labeled nucleotides
- Site-directed mutagenesis using the megaprimer method
- Site-directed mutagenesis by inverse PCR
- Creating nested DNA deletions using exonuclease III
- Transposon and transposome mutagenesis of plasmids, cosmids, and BACs
- In vitro transcription and translation
- Vectors for the expression of recombinant proteins in E. coli
- Expression of recombinant proteins form Iac promoters
- Plasmid-based reporter genes : assays for green fluorescent protein