In situ detection of DNA damage : methods and protocols /

Bibliographic Details
Other Authors:	Didenko, Vladimir V.
Format:	Book
Language:	English
Published:	Totowa, N.J. : Humana Press, [2002]
Series:	Methods in molecular biology (Clifton, N.J.) ; v. 203.
Subjects:	DNA Damage. Clinical Laboratory Techniques. DNA > analysis.

Table of Contents:

Pt. 1. Labeling DNA breaks using terminal transferase (TUNEL assay)
Labeling DNA damage with terminal transferase : applicability, specificity, and limitations
TUNEL assay : an overview of techniques
Electron microscopic detection of DNA damage labeled by TUNEL
Quantitative differentiation of both free 3' OH and 5' OH DNA ends using terminal transferase-based labeling combined with transmission electron microscopy
Determination of three-dimensional distribution of apoptotic DNA damage by combination of TUNEL and quick-freezing and deep-etching techniques
In situ detection of DNA strand breaks in analysis of apoptosis by flow- and laser-scanning cytometry
Pt. 2. Labeling DNA breaks using DNA polymerse I or its Klenow fragment
DNA damage detection using DNA polymerase I or its Klenow fragment: applicability, specificity, limitations
Labeling DNA breaks in situ by Klenow enzyme
In situ nick translation at the electron microscopic level
Pt. 3. Labeling DNA breaks using ligase
In situ DNA ligation as a method for labeling apoptotic cells in tissue sections : an overview
Detection of specific double-strand DNA breaks and apoptosis in situ using T4 DNA ligase
In situ detection of double-strand DNA breaks with terminal 5'OH groups
Pt. 4. Detection of DNA breaks in agarose trapped cells : comet assay and related techniques
The comet assay : principles, applications, and limitations
The comet assay : an overview of techniques
Ultrasensitive detection of DNA damage by the combination of the comet and TUNEL assays
Application of FISH to detect DNA damage : DNA breakage detection-FISH (DBD-FISH)
Pt. 5. Detection of modified bases and AP sites in DNA
Simultaneous in situ detection of DNA fragmentation and RNA/DNA oxidative damage using TUNEL assay and immunohistochemical labeling for 8-hydroxy-2'-deoxyguanosine (8-OHdG)
The in situ detection of apurinic/apyrimidinic sites and DNA breaks bearing extension blocking termini
Pt. 6. Indirect and general markers of DNA damage
Markers of poly (ADP-ribose) polymerase activity as correlates of DNA damage
Ultrasound imaging of apoptosis : DNA-damage effects visualized
p53 induction as an indicator of DNA damage
Detection of caspases activation in situ by fluorochrome-labeled inhibitors of caspases (FLICA).