Recombinant antibodies for cancer therapy : methods and protocols /

Bibliographic Details
Other Authors: Welschof, Martin, Krauss, Jürgen
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2003]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 207.
Subjects:
Table of Contents:
  • Generation of antibody molecules through antibody engineering
  • Application of recombinant antibodies in cancer patients
  • DNA immunization as a means to generate antibodies to proteins
  • Chimerization if a monoclonal antibody for treating hodgkin's lymphoma
  • Humanization of monoclonal antibodies by CDR grafting
  • Generation and screening of a modular human scFv expression library from multiple donors
  • Construction of semisynthetic antibody libraries
  • Single-domain VH antibody fragments from a phage display library
  • Isolation of human fab fragments against ovarian carcinoma using guided selection
  • Human recombinant fab antibodies with T-cell receptor-like specifications generated from phage display libraries
  • Engineering hot spots for affinity enhancement of antibodies
  • Simultaneous humanization and affinity optimization of monoclonol antibodies
  • Tailoring kinetics of antibodies using focused combinatorial libraries
  • Engineering scFvs for improved stability
  • Recombinant single-chain and disulfide-stabilized Fv immunotoxins for cancer therapy
  • Generation of recombinant immunotoxins for specific targeting of tumor-related peptides presented by MHC molecules
  • Construction and characterization of RNase-based targeted therapeutics
  • Bispecific diabodies for cancer therapy
  • Generation and characterization of bispecific tandem diabodies for tumor therapy
  • Generation of recombinant multimeric antibody fragments for tumor diagnosis and therapy
  • Construction and characterization of minibodies for imaging and therapy of colorectal carcinomas
  • Generation, expression, and monitoring of recombinant immune receptors for use in cellular immunotherapy
  • Tailoring natural effector functions : antibody engineering beyond humanization
  • Single-chain Fv-based affinity purification of the cellular stress protein gp96 for vaccine development
  • Recombinant adenoviruses for in vivo expression of antibody fragments
  • Production of antibody fragments in a bioreactor
  • Large scale production of recombinant antibodies by utilizing cellulose-binding domains
  • Production of tumor-specific antibodies in tobacco