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PCR mutation detection protocols /

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Bibliographic Details
Other Authors: Theophilus, Bimal D. M., Rapley, Ralph
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2002]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 187.
Subjects:
DNA Mutational Analysis > methods > Laboratory Manuals.
Polymerase Chain Reaction > methods > Laboratory Manuals.
Table of Contents:
  • Agarose and polyacrylamide gel electrophoresis
  • Internal labeling of DNA probes
  • End-labeling of DNA probes
  • Southern Blotting of agarose gels by capillary transfer
  • Restriction fragment length polymorphism
  • PCR : principles, procedures, and parameters
  • Allele-specific oligonucleotide PCR
  • Long-range PCR
  • Analysis of nucleotide sequence variations by solid-phase minisequencing
  • Cycle sequencing of PCR products
  • Fluorescent in situ hybridization
  • The protein truncation test
  • Mutation detection in factor VIII cDNA from lymphocytes of hemophilia A patients by solid phase fluorescent chemical cleavage of mismatch
  • Denaturing gradient gel electrophoresis
  • Conformation-sensitive gel electrophoresis
  • SSCP/heteroduplex analysis
  • Cleavase® fragment length polymorphism analysis for genotyping and mutation detection
  • Automated genotyping using the DNA MassArray® technology
  • An introduction to bioinformatics