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Genetic library construction and screening : advanced techniques and applications /

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Bibliographic Details
Other Authors: Bird, R. Curtis, Smith, Bruce F.
Format: Book
Language:English
Published: Berlin ; New York : Springer, [2002]
Series:Springer lab manual.
Subjects:
Cloning, Molecular > methods > Laboratory Manuals.
DNA > genetics > Laboratory Manuals.
Gene Library > Laboratory Manuals.
Polymerase Chain Reaction > methods > Laboratory Manuals.
Table of Contents:
  • Strategies for cDNA cling and mapping RNA transcripts
  • Cloning PCR products
  • Site directed deletion, insertion, and substitution using PCR
  • Long RT-PCR cloning-amplification of full length enterovirus genomes
  • Construction of cDNA libraries from small quantities of total RNA using template switching catalyzed by M-MLV reverse transcriptase
  • Subtractive hybridization and cDNA cloning
  • Differential display to identify steroid-induced genes in endocrine cells
  • Two hyrid cDNA cloning
  • Yeast one and two hyrid cDNA cloning
  • High-throughput library screening by fluorescent hybridisations on gridded membranes
  • Identification of cell targeting ligands using random peptide-presenting phage libraries.