Peptide research protocols : endothelin /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2002]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 206. |
| Subjects: |
Table of Contents:
- Immunocytochemical localization of endothelin peptides, precursors, and endothelin-converting enzymes
- Analysis of endothelins by enzyme-linked immunosorbent assay and radioimmunoassay
- Seperation of endothelin isoforms by high performance liquid chromatography and detection by radioimmunoassay
- Radioligand binding assays and quantitative autoradiography of endothelin receptors
- Using receptor antagonists in binding studies to characterize a mammalian endothelin receptor
- Quantification of endothelin receptor mRNA by competitive RT-PCR
- Detection and assessment of endothelin-converting enzyme activity
- Quantitative measurement of mRNA levels by RT-PCR: studies of ECE-1 isoforms
- Evaluation of endothelin-converting enzyme inhibitors using cultured cells
- Endothelin-converting enzyme activity in vascular smooth muscle preparations in vitro
- Characterization of a new endothelin receptor ligand by in vitro assays
- Use of in vitro organ cultures of human saphenous vein as a model for intimal proliferation
- Investigation of the endothelin system in experimental heart failure
- Local forearm vasoconstriction to endothelin-1 measured by venous occlusion plethysmography in vivo.