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Guide to cytochromes P450 : structure and function /

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Bibliographic Details
Main Author: Lewis, D. F. V.
Format: Book
Language:English
Published: London ; New York : Taylor & Francis, 2001.
Subjects:
Cytochrome P-450 Enzyme System > physiology.
Table of Contents:
  • 1. Introduction
  • Discovery of an enzyme superfamily
  • Occurrence and distribution
  • Isolation and purification
  • Characterization and detection
  • Genetic polymorphisms and allelic variants : protein sequence alignments
  • Biotechnological applications
  • Nomenclature note : cytochromes, peroxidases and P450
  • 2. Evolution of the P450 superfamily
  • P450 phylogeny
  • P450 sequences
  • Nomenclature of P450 genes and enzymes
  • 3. The P450 catalytic cycle
  • Stages in the reaction
  • Thermodynamics of the P450 reaction, rates of electron transfer and kinetics
  • Coupling of spin and redox equilibria
  • Factors influencing the rate of substrate turnover
  • Appendix - Factors influencing rates and clearance in P450 reactions : QSARs
  • 4. Substrate selectivity and metabolism
  • Endogenous substrates
  • Exogenous substrates
  • Criteria governing substrate slectivity for human hepatic P450s
  • Toxicity mediated by P450 enzymes
  • Quantitative structure-activity relationships (QSARs) in P450 substrates
  • 5. Regulation of P450 enzymes
  • Mechanisms of P450 regulation and gene expression
  • Toxic consequences of induction : predicting toxicity via the COMPACT method
  • QSARs in P450 inducers and nuclear receptor ligand binding
  • Inhibition of P450 enzymes
  • QSARs for P450 inhibitors
  • 6. The structures of cytochromes P450
  • X-ray crystallography
  • Spectroscopy
  • Site-directed mutagenesis and residue modification
  • Molecular modelling studies
  • Molecular orbital calculations
  • Conclusions.