Green fluorescent protein : applications and protocols /

Bibliographic Details
Other Authors: Hicks, Barry W.
Format: Book
Language:English
Published: Totowa, NJ : Humana Press, [2002]
Series:Methods in molecular biology ; v. 183.
Subjects:
Table of Contents:
  • Amplification of representative cDNA samples from microscopic amounts of invertebrate tissue to search for new genes
  • Use of cobA and cysGa as red fluorescent indicators
  • Circular permutation of the green fluorescent protein
  • Evolutionary design of generic green fluorescent protein biosensors
  • Random insertion of green fluorescent protein into the regulatory subunit of cyclic adenosine monophosphate-dependent protein kinase
  • Circular mRNA encoding for monomeric and polymeric green fluorescent protein
  • Fluorescence lifetime imaging (FLIM) of green fluorescent fusion proteins in living cells
  • Fluorescence resonance energy transfer (FRET) applications using green fluorescent protein : energy transfer to the endogenous chromophores of phycobilisome light-harvesting complexes
  • Bioluminescence resonance energy transfer assays for protein-protein interactions in living cells
  • Whole-body fluorescence imaging with green fluorescence protein
  • Drug-induced translocation of nucleolar proteins fused to green fluorescent protein
  • Light-induced nuclear targeting of phytochrome beta-sgreen fluorescent protein in plants
  • Mechanisms of protein trafficking : two different signal sequences fused to green fluorescent protein to study mitochondrial import
  • Analysis of nucleocytoplasmic transport using green fluorescent protein
  • Transgenic bovine embryo selection using green fluorescent protein
  • Development of glycosylphosphatidylinositol-anchored enhanced green fluorescent protein : one-step visualization of GPI fate in global tissues and ubiquitous cell surface marking
  • Transgenic zebrafish expressing green fluorescent protein
  • Transgenic insects : expressing green fluorescent protein-silk fibroin light chain fusion protein in transgenic silkworms
  • Green fluorescent protein in transgenic plants : brassica transformation
  • Green fluorescent protein calcium biosensors : calcium imaging with GFP cameleons
  • Green fluorescent protein fluobody immunosensors : immunofluorescence with GFP-antibody fusion proteins
  • Green fluorescent protein-based protein kinase biosensor substrates
  • Green fluorescent protein urea sensors : uroopathogenic Proteus mirabilis
  • Using green fluorescent protein to monitor measles virus cell-to-cell spread by time-lapse confocal microscopy
  • Tracking and selection of retrovirally transduced murine bone marrow cells using green fluorescent protein
  • Green fluorescent protein as a reporter of adenovirus-mediated gene transfer and expression in the hypothalamic-neurohypophyseal system
  • Enhancement of green fluorescent protein expression in adeno-associated virus with the woodchuck hepatitis virus post-transcriptional regulatory element
  • Construction of infectious simian vericella virus expressing green fluorescent protein
  • Green fluorescent protein in retroviral vector constructs as marker and reporter of gene expression for cell and gene therapy applications