GTPase protocols : the Ras superfamily /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2002]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 189. |
| Subjects: |
Table of Contents:
- The GTPase cycle: how dominant inhibitory mutants block the biological functions of small GTPases
- Preparation of GTPases for structural and biophysical analysis
- Using cDNA-representational difference analysis (cDNA-RDA) in combination with microarrays to identify rac regulated genes
- Fluorescence methods in the study of small GTP-binding proteins
- Ras and rac as activators of reactive oxygen species (ROS)
- Interfering with ras signaling using membrane-permeable peptides or drugs
- A ras-based module to generate P-labeled fusion proteins for blot overlays
- Determination of the activity of rho-like GTPases in cells
- Introduction of dominant inhibitory proteins directed against ROK and MRCK kinases
- Effects of rho family GTPases on cell-cell adhesion
- Cell motility and invasion assays
- Isolation of regulatory proteins for the rab3 subfamily GTPases
- Analysis and preparation of stable complexes between rab GTPases, rab escort protein, and rab geranylgeranyl transferase
- Preparation of myristoylated arf1 and arf6 proteins
- ARF-directed guanine-nucleotide-exchange (GEP) proteins
- Arf6 and its role in cytoskeletal modulation
- ARF GTPase-activating protein 1
- The use of permeabilized cell systems to study nuclear transport
- Analysis of nuclear protein import and export in vitro using fluorescent cargoes
- Characterization of the effects of ranGTP on the microtubule cytoskeleton.