Table of Contents:
  • The standard FISH procedure - an introduction
  • FISH techniques, FISH probes and their applications in medicine and biology - an overview
  • Microscopy and imaging systems
  • FISH on native, human tissues
  • FISH on chromosome preparations of peripheral blood
  • FISH on uncultured blood or bone marrow
  • FISH on blood or bone marrow smears
  • Epithelial cells from buccal smears and urine
  • FISH on hair root cells
  • Amniotic fluid cells - uncultured
  • Human sperm cells
  • FISH on archival, human tissues
  • Archival cytogenetic slides
  • Formalin-fixed and paraffin-embedded tissue sections
  • Nucleus extraction from formalin fixed/paraffin embedded tissue
  • Combined in situ nick translation and mRNA FISH for simultaneous detection of apoptosis and expression of apoptosis-related genes in sections from parafinn-embedded tissue
  • Nucleus extraction from cryofixed tissue
  • Preparation of samples for CGH by DOP-PCR from cryofixed or paraffin-embedded, microdissected cells
  • Comparative genomic hybridization
  • Special FISH techniques (Fiber-FISH, PNA-FISH, Co-FISH, NU-FISH)
  • High resolution FISH of stretched chromosome fibers
  • Quantitative DNA fiber mapping
  • PNA-FISH
  • Strand-specific fluorescence in situ hybridization: CO-FISH and COD-FISH
  • The combined use of nuclease in situ digestion and FISH (NU-FISH)
  • DNA breakage detection-FISH (DBD-FISH)
  • Detection of radiosensitivity by means of three-color FISH
  • Fluorescence in situ hybridisation of a complete set of 41 telomere specific probes in a single simultaneous hybridisation experiment using the chromoprobe multiprobe system-T
  • FISH in zoology and virology
  • Zoo-FISH
  • FISH on insect cells transfected with heterologous DNA
  • Yeast FISH: delineation of chromosomal targets in vegetative and meiotic yeast cells
  • In situ detection of viral nucleic acids by chemiluminescence
  • Multicolor FISH applications
  • Spectral imaging analysis of metaphase and interphase cells
  • Microdissection of chromosomes and reverse FISH
  • Multicolor FISH techniques for the detection of inter- and intrachromosomal rearrangements
  • Centromere-specific multicolor-FISH (cenM-FISH)
  • Simultaneous multicolor-FISH and immunocytochemical analysis of fresh tumor material
  • Simultaneous fluorescence immunophenotyping and FISH (FICTION)
  • Not FISH but almost
  • PRINS: hybridization and primed in situ labeling in one step
  • Microarrays and DNA-chips in the analysis of genetic abberations.