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Immunology of infection /

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Bibliographic Details
Other Authors: Kaufmann, S. H. E. (Stefan H. E.), Kabelitz, Dieter
Format: Book
Language:English
Published: Amsterdam ; Boston : Academic Press, [2002]
Edition:Second edition.
Series:Methods in microbiology ; v. 32.
Subjects:
Infection > immunology.
Immunity.
Table of Contents:
  • pt. I. General methods
  • 1. Phenotyping and separation of leukocyte populations based on affinity labelling
  • 2. Cytometric cytokine secretion assay : detection and isolation of cytokine-secreting T cells
  • 3. Measurement of cellular proliferation
  • 4. Use of bioinformatics to predict MHC ligands and T-cell epitopes : application to epitope-driven vaccine design
  • The use of tetramers in the quantitative analysis of T-cell responses
  • Using microarrays for studying the host transcriptional response to microbial infection
  • Genome-wide expression profiling of intracellular bacteria : the interaction of Mycobacterium tuberculosis with macrophages
  • pt. II. Murine models
  • 1. Management of immunocompromised and infected animals
  • 2. In vitro analysis
  • 3. In vivo analysis
  • 4. Specific models
  • pt. III. Human systems
  • 1. Isolation and propagation of human dendritic cells
  • 2. Isolation of T cells and establishment of T-cell lines and clones
  • 3. Growth transformation of human T cells
  • 4. Generation and characterization of human killer cells
  • 5. Measuring human cytokine responses
  • 6. Measuring immune responses in situ : immunofluorescent and immunoenzymatic techniques
  • 7. Isolation, characterization and cultivation of human monocytes and macrophages