DNA science : a first course /
| Main Author: | |
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| Other Authors: | |
| Format: | Book |
| Language: | English |
| Published: |
Cold Spring Harbor, N.Y. :
Cold Spring Harbor Laboratory Press,
[2003]
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| Edition: | Second edition. |
| Subjects: |
Table of Contents:
- How we learned that DNA is the genetic material
- How we learned the function of DNA
- How we learned how genes are regulated
- Basic tools and techniques of DNA science
- Methods for finding and expressing important genes
- Modern methods for analyzing whole genomes
- The DNA science of cancer
- Applying DNA science to human genetics and evolution
- Lab safety and adherence to national institutes of health guidelines
- Measurements, micropipetting, and sterile techniques
- Bacterial culture techniques
- DNA restrictions analysis
- Effects of DNA methylation on restriction
- Rapid colony transformation of E. coli with plasmid DNA
- Assay for an antibiotic resistance enzyme
- Purification and identification of recombinant GFP
- Purification and identification of plasmid DNA
- Recombination of antibiotic resistance genes
- Transformation of E. coli with recombinant DNA
- Replica plating to identify mixed E. coli populations
- Purification and identification of recombinant DNA