Manipulating the mouse embryo : a laboratory manual /
| Other Authors: | |
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| Format: | Book |
| Language: | English |
| Published: |
Cold Spring Harbor, N.Y. :
Cold Spring Harbor Laboratory Press,
[2003]
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| Edition: | Third edition. |
| Subjects: |
Table of Contents:
- Developmental genetics and embryology of the mouse, past, present, and future
- Summary of mouse development
- Production of transgenic and chimeric mice : general issues
- Recovery and in vitro culture of preimplantation-stage embryos
- Isolation, culture, and manipulation of postimplantation embryos
- Surgical procedures
- Production of transgenic mice
- Isolation and culture of blastocyst-derived stem cell lines
- Vector designs for embryonic stem cell-based transgenics and genome alterations
- Introduction of foreign DNA into embryonic stem cells
- Production of chimeras
- Detection and analysis of mouse genome alterations and specific sequences
- Parthenogenesis, pronuclear transfer, and mouse cloning
- Assisted reproduction : ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplamic sperm injection
- Cryopreservation, rederivation, and transport of mice
- Techniques for visualizing gene products, cells, tissues, and organ systems
- Setting up a micromanipulation lab