Superantigen protocols /

Bibliographic Details
Other Authors: Krakauer, Teresa
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2003]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 214.
Subjects:
Table of Contents:
  • Superantigens : structure, function, and diversity
  • Expression, purification, and detection of novel streptococcal superantigens
  • Flow cytometric detection of MMTV superantigens
  • Spectrophotometric methods for the determination of superantigen structure and stability
  • Binding kinetics of superantigen with TCR and MHC class II
  • Directed evolution of T-cell receptors for binding superantigens
  • Analysis of superantigen binding to soluble T-cell receptors
  • Role of accessory molecules in the superantigen-induced activation of peripheral blood T cells
  • T-lymphocyte activation induced by staphylococcal enterotoxin superantigens : analysis of protein tyrosine phosphorylation
  • Measurement of proinflammatory cytokines and T-cell proliferative response in superantigen-activated human peripheral blood mononuclear cells
  • RNase protection assay for the study of the differential effects of therapeutic agents in suppressing staphylococcal enterotoxin B-induced cytokines in human peripheral blood mononuclear cells
  • Staining protocol for superantigen-induced cytokine production studied at the single-cell level
  • Assessment of specific T-cell activation by superantigens
  • Superantigen-induced changes in epithelial ion transport and barrier function : use of in vitro model
  • Pyrogenic, lethal, and emetic properties of superantigens in rabbits and primates