Protein sequencing protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2003]
|
| Edition: | Second edition. |
| Series: | Methods in molecular biology ;
v. 211. |
| Subjects: |
Table of Contents:
- Strategies for handling polypeptides on a micro-scale
- SDS polyacrylamide gel electrophoresis for N-terminal protein sequencing
- Two-dimensional polyacrylamide gel electrophoresis for the separation of proteins for chemical characterization
- Electroblotting of proteins from polyacrylamide gels for chemical characterization
- Enzymatic cleavage of proteins
- Chemical cleavage of polypeptides
- Enzymatic preparation and isolation of glycopeptides
- Analytical and micropreparative capillary electrophoresis of peptides
- High-performance liquid chromatography on-line derivative spectroscopy for the characterization of peptides with aromatic amino acid residues
- Hydrolysis of samples for amino acid analysis
- Amino acid analysis : precolumn derivatization methods
- Post column amino acid analysis
- Amino acid analysis using pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate : analysis of hydrolyzed proteins and electroblotted samples
- Amino acid analysis in protein hydrolysates using anion exchange chromatography and IPAD detection
- D-amino acid analysis
- Validation of amino acid analysis methods
- Electrospray mass spectrometry of peptides, proteins and glycoproteins
- Peptide mass fingerprinting using MALDI-TOF mass spectrometry
- Nanoelectrospray tandem mass spectrometry and sequence similarity searching for identification of proteins from organisms with unknown genomes
- Direct identification of proteins in ultracomplex mixtures : applications to proteome analysis
- Identification of PTH-amino acids by HPLC
- Protein sequencer maintenance and troubleshooting
- N-Terminal protein sequencing for special applications
- Identification of phosphorylation sites by edman degradation
- Validation of protein sequencing in a regulated laboratory
- Automated C-terminal protein sequence analysis using the alkylated-thiohydantoin method
- Chemical modifications of proteins as an aid to sequence analysis
- Deblocking of N-terminally modified proteins
- Deblocking of proteins containing N-terminal pyroglutamic Acid
- Identification of sites of glycosylation
- Analysis of sites of protein phosphorylation
- Quantitation and location of disulfide bonds in proteins
- Getting the most from your protein sequence
- Appendix 1 : Letter codes, structures, masses, and derivatives of amino acids
- Appendix 2 : Protein consensus sequence motifs