E. coli gene expression protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2003]
|
| Series: | Methods in molecular biology ;
v. 205. |
| Subjects: |
Table of Contents:
- Cold-inducible promoters for heterologous protein expression
- Dual-expression vectors for efficient protein expression in E. coli and P. pastoris, including new modifications
- Purification of recombinant proteins from E. coli by engineered inteins
- Calmodulin-binding peptide as a removable affinity tag for protein purification
- Maltose-binding protein as a solubility enhancer
- Thioredoxin and related proteins as multifunctional fusion tags for soluble expression in E. coli
- Discovery of new fusion protein systems designed to enhance solubility in E. coli
- Assessment of protein folding/solubility in live cells
- Improving heterologous protein folding via molecular chaperone and foldase co-expression
- High-throughput purification of polyhis-tagged recombinant fusion proteins
- Co-expression of proteins in E. coli using dual expression vectors
- Small-molecule affinity-based matrices for rapid protein purification
- Use of tRNA-supplemented host strains for expression of heterologous genes in E. coli
- Studying protein-protein interactions using a bacterial two-hybrid system
- Using bio-panning of FLITRX peptide libraries displayed on E. coli cell surface to study protein-protein interactions
- Use of inteins for the in vivo production of stable cyclic peptide libraries in E. coli
- Hyperphage : improving antibody presentation in phage display
- Combinatorial biosynthesis of novell carotenoids in E. coli
- Using transcriptional-based systems for in vivo enzyme screening
- Identification of genes encoding secreted proteins using mini-ophoA mutagenesis