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Two-hybrid systems : methods and protocols /

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Bibliographic Details
Other Authors: MacDonald, Paul N.
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2001]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 177.
Subjects:
Protein Binding.
Yeasts.
Plasmids.
Laboratory Techniques and Procedures.
Table of Contents:
  • The two-hybrid system : a personal view / S. Fields ... [et al.]
  • Growth and maintenance of yeast / L.W. Bergman
  • Media formulations for various two-hybrid systems / M. Saghbini ... [et al.]
  • Yeast two-hybrid vectors and strains / P. James
  • High-efficiency transformation of plasmid DNA into yeast / R.A. Woods ... [et al.]
  • Qualitative and quantitative assessment of interactions / M.M. Montano
  • Strategies for rescuing plasmid DNA from yeast two-hybrid colonies / A. Byrd ... [et al.]
  • Two-hybrid system and false positives : approaches to detection and elimination / I.G. Serebriiskii ... [et al.]
  • Confirming yeast two-hybrid protein interactions using in vitro glutathione-S-transferase pulldowns / D.M. Kraichely ... [et al.]
  • Two-hybrid interactions confirmed by coimmunoprecipitation of epitope-tagged clones / L. Naumovski
  • Smad interactors in bone morphogenetic protein signaling / X. Yang ... [et al.]
  • Protein interactions important in eukaryotic translation initiation / K. Asano ... [et al.]
  • Steroid receptor and ligand-dependent interaction with coactivator proteins / S.A. Oñate
  • Interaction of cellular apoptosis regulating proteins with adenovirus anti-apoptosis protein E1B-19K / T. Subramanian ... [et al.]
  • Mammalian two-hybrid assays : analyzing protein-protein interactions in the transforming growth factor-beta signaling pathway / X.-H. Feng ... [et al.]
  • One-hybrid systems for detecting protein-DNA interactions / M.K. Alexander ... [et al.]
  • The split-hybrid system : uncoding multiprotein networks and defining mutations that affect protein interactions / P.S. Goldman ... [et al.]
  • Three-hybrid screens : inductible third-party systems / B. Sandrock ... [et al.]
  • Three-hybrid screens for RNA-binding proteins : proteins binding 3' end of histone mRNA / Z. Dominski ... [et al.]
  • Membrane recruitment systems for analysis of protein-protein interactions / A. Aronheim