Methods in cellular imaging /

Bibliographic Details
Corporate Author: American Physiological Society (1887- )
Other Authors: Periasamy, Ammasi
Format: Book
Language:English
Published: Oxford ; New York : Published for the American Physiological Society by Oxford University Press, 2001.
Series:Methods in physiology series.
Subjects:
Table of Contents:
  • Basics of fluorescence
  • Fluorophores and their labeling procedures for monitoring various biological signals
  • Detectors for fluorescence microscopy
  • Basics of a light microscopy imaging system and its application in biology
  • Laser scanning confocal microscopy applied to living cells and tissues
  • Diffusion measurements by fluorescence recovery after photobleaching
  • Processing microscope-acquired images for use in multimedia, print, and the World Wide Web
  • Basic principles of multiphoton excitation microscopy
  • Building a two-photon microscope using a laser scanning confocal architecture
  • Two-photon microscopy in highly scattering tissue
  • Multiphoton laser scanning microscopy and dynamic imaging in embryos
  • In vivo diffusion measurements using multiphoton excitation fluorescence photobleaching recovery and fluorescence correlation spectroscopy
  • Cellular response to laser radiation in fluorescence microscopes
  • Measurement of fluorescence resonance energy transfer in the optical microscope
  • Frequency-domain fluorescence lifetime imaging microscopy: a window on the biochemical landscape of the cell
  • Wide-field, confocal, two-photon, and lifetime resonance energy transfer imaging microscopy
  • One- and two-photon confocal fluorescence lifetime imaging and its applications
  • Biological applications of time-resolved, pump-probe fluorescence microscopy and spectroscopy in the frequency domain
  • Spectral microscopy for quantitative cell and tissue imaging
  • Total internal reflection fluorescence microscopy
  • Laser traps in cell biology and biophysics
  • Bioluminescence imaging of gene expression in living cells and tissues
  • Imaging living cells and mapping their surface molecules with the atomic force microscope.