Genomic imprinting : methods and protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2002]
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 181. |
| Subjects: |
Table of Contents:
- Generation of monoparental embryos for investigation into genomic imprinting
- Deriving and propagating mouse embryonic stem cell lines for studing genomic imprinting
- Balanced translocation for the analysis of imprinted regions of the mouse genome
- Production of YAC transgenic mice by pronuclear injection
- A transgenic approach to studying imprinted genes : modified BACs and PACs
- Methylation-sensitive genome scanning
- Subtraction-hybridization method for the identification of imprinted genes
- Identification of imprinted loci by methylation : use of methylation-sensitive representational difference analysis (Me-RDA)
- Ribonuclease protection
- Quantitative RT-PCR-based analysis of allele-specific gene expression
- Allele-specific in situ hybridization (ASISH)
- RNA-FISH to analyze allele-specific expression
- Flow cytometry and FISH to investigate allele-specific replication timing and homologous association of imprinted chromosomes
- Southern analysis using methyl-sensitive restriction enzymes
- A PCR-based method for studing DNA methylation
- Bisulfite-based methylation analysis of imprinted genes
- Direct analysis of chromosome methylation
- In vitro methylation of predetermined regions in recombinant DNA constructs by excision and religation
- Detection of methyl-sensitive DNA-binding proteins with possible involvement in the imprinting phenomenon
- Probing chromatin, structure with nuclease sensitivity assays
- Examining histone acetylation at specific genomic regions
- Purification of the MeCP2/histone deacetylase complex from Xenopul laevis
- Reconstitiution of chromatin in vitro
- Genomic imprinting in plants.