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Gene probes : principles and protocols /

Bibliographic Details
Other Authors: Aquino de Muro, Marilena, Rapley, Ralph
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2002]
Series:Methods in molecular biology ; 179.
Subjects:
Nucleic Acid Probes.
Laboratory Manual.
Table of Contents:
  • Target format and hybridization conditions
  • End labeling procedures : an overview
  • Photobiotin labeling
  • Nick translation
  • Random primed labeling
  • Design and evaluation of 16S rRNA-targeted oligonucleotide probes for fluorescence in situ hybridization
  • Evaluation of alternations in the tumor suppressor genes INK4A and INK4B in human bladder tumors
  • The beta-tubulin gene region as a molecular marker to distinguish Leishmania parasites
  • Detection of malignant plasma cells in the bone marrow and peripheral blood of patients with multiple myeloma
  • Detection of Chlamydia trachomatis by DNA amplification
  • Isolation and identification of Campylobacter species by use of selective enrichment, nucleic acid amplification methods, and gene probes
  • Diagnosis of human papillomavirus using in situ hybridization and in situ polymerase chain reaction
  • Quantitation of in situ hybridization using image analysis of radioactively labeled RNA probes
  • Stable isotope probing for detection of methanotrophs after enrichment with ¹³CH₄
  • Detection of Enterococci in freshwater and seawater (16S and 23S rRNA Enterococci Oligonucleotide Probes)
  • Analysis of 16S-23S rRNA intergenic spacer of Vibrio cholerae and Vibrio mimicus for detection of these species
  • Colony hybridization of bacterial isolates with Burkholderia cepacia-specific probes
  • In situ hybridization of Burkholderia cepacia cells
  • Detection of polychlorinated biphenyl-degrading organisms in soil
  • Detection of single-copy genes in DNA from transgenic plants
  • Differentiation between transcripts of genes belonging to small families during fruit ripening and abscission
  • Polymerase chain reaction detection of invasive Shigella and Salmonella enterica in food
  • Polymerase chain reaction for detection of Listeria monocytogenes.