Embryonic stem cells : methods and protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2002]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 185. |
| Subjects: |
Table of Contents:
- Methods for the isolation and maintenance of murine embryonic stem cells
- The use of a chemically defined media for the analyses of early development in ES cells and mouse embryos
- Analysis of the cell cycle in mouse embryonic stem cells
- Murine embryonic stem cells as a model for stress proteins and apoptosis during differentiation
- Effects of altered gene expression on ES cell differentiation
- Hypoxic gene regulation in differentiating ES cells
- Regulation of gap protein (connexin) genes and function in differentiating ES cells
- Embryonic stem cell differentiation as a model to study hematopoietic and endothelial cell development
- Analysis of Bcr-Abl function using an in vitro embryonic stem cell differentiation system
- Embryonic stem cells as a model for studying osteoclast lineage development
- Differentiation of embryonic stem cells as a model to study gene function during the development of adipose cells
- Embryonic stem cell differentiation and the vascular lineage
- Embryonic stem cells as a model to study cardiac, skeletal muscle, and vascular smooth muscle cell differentiation
- Cardiomyocyte enrichment in differentiating ES cell cultures : strategies and applications
- Embryonic stem cells as a model for the physiological analysis of the cardiovascular system
- Isolation of lineage-restricted neuroal precursors from cultured ES cells
- Lineage selection for generation and amplification of neural precursor cells
- Selective neural induction from ES cells by stromal cell-derived inducing activity and its potential therapeutic application in Parkinson's disease
- Epidermal lineage
- ES cell differentiation into the hair follicle lineage in vitro
- Embryonic stem cells as a model for studying melanocyte development
- Using progenitor cells and gene chips to define genetic pathways
- ES cell-mediated conditional transgenesis
- Switching on lineage tracers using site-specific recombination
- From ES to mice : the gene trap approach
- Functional genomics by gene-trapping in embryonic stem cells
- Phage-displayed antibodies to detect cell markers
- Gene transfer using targeted filamentous bacteriophave
- Single-cell PCR methods for studying stem cells and progenitors
- Nonradioactive labeling and detection of mRNAs hybridized onto nucleic acid cDNA arrays
- Expression profiling using quantitative hybridization on microarrays
- Isolation of antigen-specific intracellular antibody fragments as single chain Fv for use in mammalian cells
- Detection and visualization of protein interactions with protein fragment complementation assays
- Direct selection of cDNAs by phage display
- Screening for protein-protein interactions in the yeast two-hybrid system