Calcium-binding protein protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2002]
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 172. Methods in molecular biology (Clifton, N.J.) ; v. 173. |
| Subjects: |
Table of Contents:
- v. 1. Reviews and case studies
- Calcium-binding proteins
- Calcium
- Crystal structure of calpain and insights into Ca²⁺-dependent activation
- The multifunctional S100 protein family
- Ca²⁺-binding to proteins containing gamma-carboxyglutamic acid residues
- The caseins of milk as calcium-binding proteins
- Preparation of recombinant plant calmodulin isoforms
- Isolation of recombinant cardiac troponin C
- Skeletal muscle troponin C : expression and purification of the recombinant intact protein and its isolated N- and C-domain fragments
- Pruificationof recombinant calbindin D9k
- S100 proteins : from purification to functions
- Cadherins
- Alpha-cactabumin and (calcium-binding) lysozyme
- Recombinant annexin II tertramer
- Purification and characterization of ALG-2 : a novel apoptosis-linked Ca²⁺-binding protein
- Crystalilization and structural details of Ca²⁺-induced conformational changes in the EF-hand domain VI of calpain
- Neurocalcin : role in neuronal signaling
- Crystallization and structure-function of calsequestrin
- Use of fluorescence resonance energy transfer to monitor Ca²⁺-triggered membrane docking of C2 domains
- Ca²⁺-binding mode of the C₂A-domain of synaptotagmin
- Study of calcineurin structure by limited peoteolysis
- v. 2. Methods and techniques
- Quantitative analysis of Ca²⁺-binding by flow dialysis
- Calcium binding to proteins studies via competition with chromophoric chelators
- Deconvolution of calcium-binding curves : facts and fantasies
- Absorption and circular dichroism spectroscopy
- Fourier transform infrared spectroscopy of calcium-binding proteins
- Steady-state fluorescence spectroscopy
- Fluorescence methods for measuring calcium affinity and calcium exchange with proteins
- Surface plasmon resonance of calcium-binding proteins
- Differential scanning calorimetry
- Isothermal titration calorimetry
- Multiangle laser light scattering and sedimentation equilibrium
- Small-angle solution scattering reveals information on conformational dynamics in calcium-binding proteins and in their interactions with regulatory targets
- Investigation of calcium-binding proteins using electrospray ionization mass spectrometry
- Synthetic calcium-binding peptides
- Proteolytic fragments of calcium-binding proteins
- Electron magnetic resonance studies of calcium-binding proteins
- Cadmium-113 and Lead 207 NMR spectroscopic studies of calcium-binding proteins
- Calcium-43 of NMR of calcium-binding proteins
- Exploring familial relationships using multiple sequence alignment
- Structure determination by NMR : isotope labeling
- Protein structure calculation from NMR data
- Shape and dynamics of a calcium-binding protein investigated by Nitrogen-15 NMR relaxation
- The use of dipolar couplings for the structure refinement of a pair of calcium-binding EGF domains
- Vector geometry mapping : a method to characterize the confromation of helix-loop-helix calcium-binding proteins
- Use of calmodulin antagonists and S-100 protein interacting drugs for affinity chromatography
- Enzymatic assays to compare calmodulin isoforms, mutants, and chimeras
- Gene expression in transfected cells
- Monitoring the intracellular free Ca²⁺-calmodulin concentration with genetically-encoded fluorescent indicator proteins
- Studying the spatial distribution of Ca²⁺-binding proteins : how does it work for calmodulin