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Epstein-Barr virus protocols /

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Bibliographic Details
Other Authors: Wilson, Joanna B., May, Gerhard H. W.
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2001]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 174.
Subjects:
Herpesvirus 4, Human > physiology.
Herpesvirus 4, Human > isolation & purification.
Viral Proteins > chemistry.
Viral Proteins > genetics.
Virus Cultivation > methods.
Immunoassay > methods.
Table of Contents:
  • Epstein-Barr virus : the B95-8 strain map / P.J. Farrell
  • Analysis of replication of oriP-based plasmids by quantitative, competitive PCR / A.L. Kirchmaier
  • Genetic analysis and gene expression with mini-Epstein-Barr virus plasmids / E. Kilger ... [et al.]
  • Construction of cDNA libraries for the analysis of the structure of complementary strand transcripts (CSTs) / P.R. Smith
  • Analysis of the expression and function of the EBV-encoded small RNAs, the EBERs, in heterologous cells / K.G. Laing ... [et al.]
  • Visualizing EBV expression patterns by FISH / A. Szeles
  • In situ detection of Epstein-Barr virus DNA and viral gene products / G. Niedobitek ... [et al.]
  • Phenotype determination of Epstein-Barr virus-infected cells in tissue sections / H. Herbst ... [et al.]
  • Detection of EBV infection at the single-cell level : precise quantitation of virus-infected cells in vivo / G.J. Babcock ... [et al.]
  • Detection and discrimination of latent and replicative herpesvirus infection at the single cell level in vivo / L.L. Decker ... [et al.]
  • Virus isolation / L.M. Hutt-Fletcher ... [et al.]
  • Generation of lymphoblastoid cell lines (LCLs) / T. Frisan ... [et al.]
  • Introduction of plasmid vectors into cells via electroporation / A.L. Kirchmaier
  • Malignant transformation and immortalization assays in animal cells transfected with the BARF1 gene / T. Ooka
  • Transient gene expression and MACS enrichment / D. Kube ... [et al.]
  • In vitro assays to study epithelial cell growth / C.W. Dawson ... [et al.]
  • In vitro assays to study epithelial cell differentiation / C.W. Dawson ... [et al.]
  • Cell sensitivity assays : quantitative detection of apoptotic cells in vitro using the TUNEL assay / N.A. Jones ... [et al.]
  • Qualitative detection of apoptotic cells assessed by DNA fragmentation / A.J. McIlwrath
  • Regression assay / T. Frisan ... [et al.]
  • Generation of polyclonal EBV-specific CTL cultures and clones / V. Levitsky ... [et al.]
  • Determination of antigen and fine peptide specificity of EBV-specific CTLs / V. Levitsky ... [et al.]
  • Limiting dilution assay / T. Frisan ... [et al.]
  • Antibodies for detecting EBV latent proteins / M. Rowe ... [et al.]
  • Detection of EBV latent proteins by Western Blotting / M. Rowe ... [et al.]
  • Biosynthetic radiolabeling of virus glycoproteins for immunoprecipitation and electrophoretic analysis / L.M. Hutt-Fletcher ... [et al.]
  • The yeast two-hybrid assay to identify interacting proteins / K.M. Izumi
  • Identification of transactivation, repression, and protein-protein interaction domains using GAL4-fusion proteins / J.J.-D. Hsieh ... [et al.]
  • Magnetic DNA affinity purification of a cellular transcription factor / L.J. Strobi ... [et al.]
  • Screening an expression library with a DNA binding site / Q. Zhang ... [et al.]
  • Analysis of DNA binding proteins by mobility shift assay / Y.-C.J. Wang ... [et al.]
  • Analysis of RNA-protein interactions of the EBV-encoded small RNAs, the EBERs : in vitro assays / K.G. Laing ... [et al.]
  • Chimeric and mutated variants of LMP1 : a helpful tool to analyze the structure-function relationship of a pseudoreceptor / O. Gires ... [et al.]
  • Assaying the activity of kinases regulated by LMP1 / A. Kieser
  • In vitro assays for the detection of protein tyrosine phosphorylation and protein tyrosine kinase activities / S. Fruehling ... [et al.]
  • Analysis of apoptosis in tissue sections / V. Save ... [et al.]
  • Considerations in generating transgenic mice : DNA, RNA, and protein extractions from tissues : rapid and effective blotting / J.B. Wilson ... [et al.]
  • In vivo assay of cellular proliferation / J. Curran
  • Topical chemical carcinogen treatment in mice / J. Curran
  • Separation of epidermal tissue from underlying dermis and primary keratinocyte culture / J. Macdiarmid ... [et al.]
  • Selection and enrichment of B cells from lymphoid tissues / P. Tsimbouri ... [et al.]
  • Detection of immunoglobulin gene rearrangements / R.G. Caldwell ... [et al.]